Journal: PLoS ONE

Article Title: Identification and Comparative Analysis of the Tegillarca granosa Haemocytes MicroRNA Transcriptome in Response to Cd Using a Deep Sequencing Approach

doi: 10.1371/journal.pone.0093619

Figure Lengend Snippet: Sequences of primers used in this study for qRT-PCR.

Article Snippet: NCode miRNA First-Strand cDNA Synthesis and qRT-PCR Kits (Ambion, USA) was used for polyadenylation and reverse transcription of miRNAs for use in two-step quantitative RT-PCR.

Techniques:

Journal: PLoS ONE

Article Title: Identification and Comparative Analysis of the Tegillarca granosa Haemocytes MicroRNA Transcriptome in Response to Cd Using a Deep Sequencing Approach

doi: 10.1371/journal.pone.0093619

Figure Lengend Snippet: A. Fold change of five miRNAs that were differentially expressed between C and E library based on deep sequencing data. B. The relative expression abundance of the five miRNAs in haemocytes at challenge with the gradient concentration of 25, 250 and 500 μg/L Cd 2+ by qRT-PCR. * P <0.05, ** P <0.01. The amount relative to the internal control U6 is expressed as mean ± SD (N = 3).

Article Snippet: NCode miRNA First-Strand cDNA Synthesis and qRT-PCR Kits (Ambion, USA) was used for polyadenylation and reverse transcription of miRNAs for use in two-step quantitative RT-PCR.

Techniques: Sequencing, Expressing, Concentration Assay, Quantitative RT-PCR